Effect of formalin pigment removal on peroxidase-antiperoxidase immunoperoxidase technique.
نویسندگان
چکیده
منابع مشابه
Effect of formalin pigment removal on peroxidase-antiperoxidase immunoperoxidase technique.
In immunoperoxidase preparations formalin pigment (acid formaldehyde haematein) may be difficult to distinguish from the brown staining of the 3,3'-diaminobenzidine (DAB) reaction product. The pigment, produced when formalin is in contact with blood rich tissues, takes the form of dark brown microcrystals that have the ability to rotate the plane of polarised light.' Even though formalin pigmen...
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The effect of including sodium azide as a bacteriostatic agent in solutions used to dilute antibodies conjugated with the enzyme horseradish peroxidase was examined. An enzyme-linked immunosorbent assay (ELISA) and an immunohistochemical method were used and both techniques demonstrated an inhibitory effect of sodium azide on the activity of the peroxidase conjugates. It is concluded that the u...
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ژورنال
عنوان ژورنال: Journal of Clinical Pathology
سال: 1986
ISSN: 0021-9746
DOI: 10.1136/jcp.39.8.923